CircRTN4 inhibits the progression of gastric cancer by sponging miR-424-5p and regulating LATS2 expression

Purpose: To determine the expression of circRTN4 in gastric cancer (GC) and its role tumor progression.
 Methods: GEO dataset GSE93541 was analyzed using GEO2R. Starbase website used to predict combination miRNA circRTN4. The relationship between prognosis Kaplan-Meier Plotter database, while levels circRTN4, miR-424-5p, LATS2 were assessed by quantitative real time-polymerase chain reaction (qRT-PCR). CCK8, EDU, Transwell, Western blot assess GC proliferation, migration, invasion, stemness. Lastly, co-transfection miR-424-5p or si-LAST2 reversely demonstrate regulatory effect on progression cells. Significantly downregulated screened, combined downstream gene predicted Starbase.
 Results: average relative mRNA tissues significantly lower than adjacent tissues. MiR-424-5p highly expressed tissues, decreased (p < 0.05). Low associated with a low survival rate patients. pLCDH- inhibited proliferation cells Overexpression migration invasion cells, pLCDH-circRTN4 reduced ability stem expressions MMP2 OCT4.
 Conclusion: Expression decreases GC, contributes development this disease increasing binding miR-424-5p. This suggests that may serve as promising prognostic marker well potential therapeutic target for